Journal: Nature Communications
Article Title: A humanized mouse model for adeno-associated viral gene therapy
doi: 10.1038/s41467-024-46017-0
Figure Lengend Snippet: Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. Immunohistochemistry showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.
Article Snippet: Immunofluorescence (IF) was developed using fluorescent-labeled secondary antibodies (Jackson ImmunoResearch Laboratories) and IHC was developed following Vector ImmPRESS Duet immunohistochemistry kit manufacturer’s instructions (Vector Labs, cat# MP-7714–15).
Techniques: Injection, Expressing, Immunohistochemistry, Staining